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KMID : 0545120110210121294
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 12 p.1294 ~ p.1298
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Cloning of metK from Actinoplanes teichomyceticus ATCC31121 and Effect of Its High Expression on Antibiotic Production
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Kim Du-Yeong
Hwang Yong-Il
Choi Sun-Uk
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Abstract
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A metK gene encoding S-adenosyl-L-methionine synthetase was cloned from the non-Streptomyces actinomycetes, Actinoplanes teichomyceticus ATCC31121. In order to evaluate the effect of the metK expression on antibiotic production in actinomycetes, an expression vector harboring the metK gene was constructed and introduced into Streptomyces lividans TK24 and A. teichomyceticus, and the antibiotic production of the exconjugants was assessed. As a result, it was determined that the expression of metK induced 17-fold and 2.2-fold increases in actinorhodin production from S. lividans TK24 and teicoplanin production from A. teichomyceticus, respectively, compared with the control strains.
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KEYWORD
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Actinoplanes teichomyceticus, teicoplanin, metK, cloning, actinorhodin
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